Studies on the germination of Bacillus anthracoides spores induced by nutrient germinant / 中华流行病学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To explore the germination effects of Bacillus anthracoides spores germinant to nutrient germinant.</p><p><b>METHODS</b>Heat factors and nutrient germinant were used to stimulate the Bacillus anthracoides spores and to germinate. Ultraviolet spectrophotometer was used to measured the A value of spore solution in the wavelength of 600 nm. Accrding to the A value, the germination rates in different condition. Transmission electron microscope was used to observe the ultrastructure changes of spores.</p><p><b>RESULTS</b>The rate of germination effects were 68.0% under 6 mmol/L inosine at 37 degrees C, pH 7.9; 74.5% under 70 mmol/L L-alanine at 30 degrees C, pH 8.9; and 85.6% under 6 mmol/L inosine and 70 mmol/L L-alanine at 37 degrees C, pH 8.2. Under transmission electron microscope, the germinated spores' coat and cortex were brokendown and degraded with its core completely exposed.</p><p><b>CONCLUSION</b>Under suitable environment, the nutrient germinant with inosine and L-alanine might be helpful for germinating the bacillus anthracoides spores.</p>