Expression pattern of membrane type-1 matrix metalloproteinase in primary breast carcinomas / 南方医科大学学报
Journal of Southern Medical University
;
(12): 94-97, 2016.
Artículo
en Chino
| WPRIM
| ID: wpr-232504
ABSTRACT
<p><b>OBJECTIVE</b>To examine the expression pattern of membrane type-1 matrix metalloproteinase (MT1-MMP) in breast carcinomas.</p><p><b>METHODS</b>Forty-three breast cancer tissues were collected and examined for MT1-MMP protein and mRNA expressions using immunohistochemistry, semi-quantitative RT-PCR and in situ hybridization.</p><p><b>RESULTS</b>Immunohistochemistry of the breast cancer specimens showed MT1-MMP immunoreactivity on the cancer cell membrane. MT1-MMP mRNA was located in the stromal cells surrounding the breast cancer nest as shown by in situ hybridization. MT1-MMP mRNA expression was detected in all of the carcinomas, but its level was significantly lower in immunohistochemically negative specimens than in positive ones (0.547=0.0886 vs 0.759=0.0802, Plt;0.01).</p><p><b>CONCLUSION</b>MT1-MMP is very likely produced by stromal cells surrounding the breast cancer nest and anchored on the cell membrane after activation.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Neoplasias de la Mama
/
ARN Mensajero
/
Inmunohistoquímica
/
Células Tumorales Cultivadas
/
Hibridación in Situ
/
Metaloproteinasa 14 de la Matriz
/
Genética
/
Metabolismo
Límite:
Femenino
/
Humanos
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2016
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS