Evaluation of different pathological staining methods for classifying renal amyloidosis / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1448-1452, 2013.
Artículo
en Chino
| WPRIM
| ID: wpr-232777
ABSTRACT
<p><b>OBJECTIVE</b>To analyze the effect of special staining and immunohistochemical staining in distinguishing the types of renal amyloidosis to improve the diagnosis accuracy.</p><p><b>METHODS</b>Congo red staining with different methods, and immunohistochemical staining of Kappa, Lambda and Amyloid A with different antigen retrieval methods were used for staining frozen and paraffin-embedded renal tissue sections.</p><p><b>RESULTS</b>Wright's Congo red staining produced a better contrast and a higher resolution and showed a greater stability than the other 2 methods after repeated use for staining the renal tissue sections (P<0.05). Immunofluorescent staining produced better results in frozen renal tissue sections than in paraffin-embedded tissues. Immunofluorescent staining produced had better performance than immunohistochemical staining in paraffin-embedded tissues. The retrieval effect with protein kinase K was the best among the antigen retrieval methods in paraffin-embedded tissues.</p><p><b>CONCLUSION</b>Wright's Congo red staining is better than the other 2 methods in diagnosing renal amyloidosis. Immunohistochemical staining of Kappa, Lambda and Amyloid A in frozen renal tissue sections is necessary to discriminate the types of renal amyloidosis. For paraffin-embedded renal tissues, antigen retrieval using protein kinase K is better than the other 2 methods.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Coloración y Etiquetado
/
Inmunohistoquímica
/
Técnica del Anticuerpo Fluorescente
/
Clasificación
/
Adhesión en Parafina
/
Rojo Congo
/
Diagnóstico
/
Colorantes
/
Secciones por Congelación
Tipo de estudio:
Estudio diagnóstico
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2013
Tipo del documento:
Artículo
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