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Phenotypic analysis of luxS gene deletion mutants and its application in virulence regulation research in group B Streptococcus / 南方医科大学学报
Journal of Southern Medical University ; (12): 117-121, 2006.
Artículo en Chino | WPRIM | ID: wpr-232808
ABSTRACT
<p><b>OBJECTIVE</b>To explore the mechanism of virulence regulation in group B streptococcus (BGS) by studying LuxS-related AI-2 quorum-sensing pathway in GBS.</p><p><b>METHODS</b>luxS gene deletion mutants of GBS (Delta lusX) were characterized by reverse transcription (RT)-PCR, colony immunoblot analysis, growth curve measurement, and cAMP determination. Functional analysis of luxS in the deletion mutants was conducted by bioluminescence assay.</p><p><b>RESULTS</b>Genetic analysis results showed that the luxS deletion in the mutant 515-Delta lusX caused upregulation of scpB gene expression. Phenotypic analysis revealed that, in comparison with the wild strain, 515-Delta lusX mutant grew slowly in DCM media but quickly in THY media. An approximately two-fold decrement in bioluminescence was detected in the luxS deletion mutants as compared with the wild strain.</p><p><b>CONCLUSION</b>This study confirms the importance of LuxS molecule in the AI-2 quorum-sensing pathway in GBS and provides new insights into the virulence regulation mechanism of GBS.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Fenotipo / Streptococcus agalactiae / Liasas de Carbono-Azufre / Proteínas Bacterianas / Virulencia / Iluminación / Regulación Bacteriana de la Expresión Génica / Eliminación de Gen / Genética Idioma: Chino Revista: Journal of Southern Medical University Año: 2006 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Fenotipo / Streptococcus agalactiae / Liasas de Carbono-Azufre / Proteínas Bacterianas / Virulencia / Iluminación / Regulación Bacteriana de la Expresión Génica / Eliminación de Gen / Genética Idioma: Chino Revista: Journal of Southern Medical University Año: 2006 Tipo del documento: Artículo