Establishment of miniCTTA multiplex set with fluorescence-labeled primers / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1574-1576, 2006.
Artículo
en Chino
| WPRIM
| ID: wpr-232834
ABSTRACT
<p><b>OBJECTIVE</b>To establish a miniCTTA multiplex set including short tandem repeat (STR) markers as CSF1PO, TH01, and TPOX and amelogenin gene, whose amplified fragments are shorter than 130 bp.</p><p><b>METHODS</b>The length of the gene fragments amplified with fluorescence-labeled primers were analyzed by ABI3100 Genetic Analyzer for genotyping of 100 unrelated individuals, 10 genealogies and 30 highly degraded specimens.</p><p><b>RESULTS</b>The genotypes derived form miniCTTA were consistent with the results by AmpFLSTR Identifiler kit.</p><p><b>CONCLUSION</b>MiniCTTA multiplex set is useful for personal identification and paternity test, especially applicable for degraded DNA sample.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
ADN
/
Secuencia de Bases
/
Marcadores Genéticos
/
Reproducibilidad de los Resultados
/
Dermatoglifia del ADN
/
Hibridación Fluorescente in Situ
/
Cartilla de ADN
/
Repeticiones de Microsatélite
/
Técnicas de Amplificación de Ácido Nucleico
/
Amelogenina
Tipo de estudio:
Estudio pronóstico
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2006
Tipo del documento:
Artículo
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