Determination of propofol in human serum by improved reversed phase high-performance liquid chromatography with fluorescence detection / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To develop a new high-performance liquid chromatographic (HLPC) method for determination of propofol in human serum.</p><p><b>METHODS</b>Human serum samples were precipitated with 20% perchloric acid and centrifuged to obtain 50 microl of the supernatant for analysis by HPLC coupled with fluorescence detection. The analysis was performed with a C(18) reversed-phase column using a acetonitrile-water (9010) phase delivered at 1.0 ml/min, with the excitation wavelength of 276 nm and emission wavelength of 310 nm.</p><p><b>RESULTS</b>The calibration curves were linear (r=0.997 5) within the concentration range of 0.05-10 microg/ml, the limit of propofol quantification was 50 ng/ml and the intra- and inter-day precisions were between -/+15%.</p><p><b>CONCLUSIONS</b>The method is accurate, sensitive and simple for propofol determination in clinical anesthesia.</p>