Rapid detection of Listeria monocytogenes by immunomagnetic separation combined with selective medium / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 672-680, 2013.
Artículo
en Chino
| WPRIM
| ID: wpr-233210
ABSTRACT
Listeria monocytogenes is a pathogenic bacterium, therefore, it is essential for food safety monitoring to establish a rapid and specific detecting method. In this study, immunomagnetic beads and selective medium were combined to detect Listeria monocytogenes at different concentrations (10(1)-10(5) CFU/mL). Other three types of Listeria spp., Staphylococcus aureus and Vibrio parahaemolyticus were also detected to conduct the cross-reaction analysis. Meanwhile, contaminated milk samples were prepared to explore the limit of detection of immunomagnetic beads combining with selective medium. Results showed that Listeria monocytogenes with the concentration of 10(3) CFU/mL and above was successfully detected. Milk samples were detected within 6 hours, with a detection limit of 0.7 CFU/mL. The method developed is capable of detecting milk samples within 30 h, which is 38 h faster compared with national standard method with the same sensitivity.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Química
/
Técnicas Bacteriológicas
/
Sensibilidad y Especificidad
/
Separación Inmunomagnética
/
Medios de Cultivo
/
Listeria monocytogenes
/
Métodos
Tipo de estudio:
Estudio diagnóstico
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2013
Tipo del documento:
Artículo
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