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Heterologous expression and enzymatic analysis of Streptomyces griseus trypsin in Streptomyces lividans / 生物工程学报
Chinese Journal of Biotechnology ; (12): 466-479, 2013.
Artículo en Chino | WPRIM | ID: wpr-233229
ABSTRACT
Trypsin as an important serine protease has been widely used in food, pharmaceutical and tanning industries. In this study, we successfully expressed trypsin (cloning from Streptomyces griseus ATCC10137) in Streptomyces lividans TK24 and comparatively investigated its enzymatic properties. Specifically, applying S. griseus ATCC 10137 genome as template, we obtained the sprT gene and sub-cloned it into the expression plasmid pIJ86, generating the recombinant strain S. lividans TK24/pIJ86-sprT. When cultivated in R2YE and SELF, the activity of rSGT reached 9.21 U/mL and 8.61 U/mL, respectively. Meanwhile, the results of the enzymatic analysis showed that rSGT exhibited a higher acid tolerance and a higher specificity to hydrolyze amide bonds compared with bovine trypsin (BT). In addition, Zn2+ and organic solvents up-regulated esterase and amidase of rSGT. Taken together, the results obtained herein provide meaningful information for further modification of rSGT and its industrial application.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Streptomyces griseus / Proteínas Recombinantes / Tripsina / Streptomyces lividans / Fermentación / Genética / Metabolismo Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2013 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Streptomyces griseus / Proteínas Recombinantes / Tripsina / Streptomyces lividans / Fermentación / Genética / Metabolismo Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2013 Tipo del documento: Artículo