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Expression of immune response molecules and function of fas ligand on surface of AML WEHI-3 cells / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 535-538, 2006.
Artículo en Chino | WPRIM | ID: wpr-233551
ABSTRACT
The purpose of this study was to investigate the expression of Fas, Fas ligand (FasL) and CD80 and function of FasL on the surface of acute myelomonocytic leukemia cells from WEHI-3 line. The expression of Fas, FasL and CD80 on the surface of WEHI-3 were detected by flow cytometry, the apoptosis of YAC-1 cell induced by FasL on the surface of WEHI-3 were detected by (3)H-TdR incorporation. The results showed that the expression rate of Fas, FasL and CD80 on the surface of WEHI-3 cells were (6.75 +/- 2.31)% (n = 5), (63.73 +/- 5.23)% (n = 5) and (5.06 +/- 0.41)% (n = 5) respectively. The apoptosis rate of YAC-1 cells (target cells) co-cultured with WEHI-3 cells (Effector cells) at the rate of 13, 110 and 130 were (26 +/- 4.5)%, (35 +/- 3.2)% and (43 +/- 2.7)% (n = 5) respectively. It is concluded that WEHI-3 cells have high expression of FasL and low expression of Fas and CD80 on their cell membrane, and can induce the apoptosis of Fas(+) YAC-1 cells.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Patología / Fisiología / Células Tumorales Cultivadas / Leucemia Mielomonocítica Aguda / Membrana Celular / Apoptosis / Antígeno B7-1 / Receptor fas / Proteína Ligando Fas / Metabolismo Límite: Humanos Idioma: Chino Revista: Journal of Experimental Hematology Año: 2006 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Patología / Fisiología / Células Tumorales Cultivadas / Leucemia Mielomonocítica Aguda / Membrana Celular / Apoptosis / Antígeno B7-1 / Receptor fas / Proteína Ligando Fas / Metabolismo Límite: Humanos Idioma: Chino Revista: Journal of Experimental Hematology Año: 2006 Tipo del documento: Artículo