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Recombinant adenovirus-mediated expression of 1.2-copy hepatitis B virus DNA in three hepatocytes / 南方医科大学学报
Journal of Southern Medical University ; (12): 738-740, 2009.
Artículo en Chino | WPRIM | ID: wpr-233695
ABSTRACT
<p><b>OBJECTIVE</b>To study hepatitis B virus (HBV) expression in 3 hepatocytes infected with recombinant adenovirus containing 1.2-copy HBV DNA.a</p><p><b>METHODS</b>A chicken hepatoma cell line and two human hepatocytes were infected by the recombinant adenovirus containing 1.2-copy HBV DNA at 25 pfu/cell. HBV-specific mRNA was detected by RT-PCR 3 days after the infection, and HBsAg and HBeAg were detected by ELISA and HBV DNA by real-time PCR daily after the infection.</p><p><b>RESULTS</b>HBV mRNA expression was detected in all the 3 cells after recombinant adenovirus infection, and the quantities of HBV DNA and HBV antigens in the culture supernatant increased with the passage of time.a</p><p><b>CONCLUSION</b>Infection with the recombinant adenovirus containing 1.2-copy HBV DNA can mediate HBV infection in the 3 cells in vitro.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Virología / ADN Recombinante / ADN Viral / Expresión Génica / Adenoviridae / Virus de la Hepatitis B / Medios de Cultivo Condicionados / Reacción en Cadena de la Polimerasa de Transcriptasa Inversa / Hepatocitos / Línea Celular Tumoral Límite: Animales / Humanos Idioma: Chino Revista: Journal of Southern Medical University Año: 2009 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Virología / ADN Recombinante / ADN Viral / Expresión Génica / Adenoviridae / Virus de la Hepatitis B / Medios de Cultivo Condicionados / Reacción en Cadena de la Polimerasa de Transcriptasa Inversa / Hepatocitos / Línea Celular Tumoral Límite: Animales / Humanos Idioma: Chino Revista: Journal of Southern Medical University Año: 2009 Tipo del documento: Artículo