A simple and efficient method for establishing a mouse model of orthotopic MB49 bladder cancer / 南方医科大学学报
Journal of Southern Medical University
;
(12): 627-630, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-233725
ABSTRACT
<p><b>OBJECTIVE</b>To establish a simple and efficient method for establishing a mouse model of orthotopic superficial bladder cancer.</p><p><b>METHODS</b>C57BL/6 mice were anesthetized with sodium pentobarbital and catheterized with modified IV catheter (24 G). The mice were intravesically pretreated with HCl and then with NaOH, and after washing the bladders with phosphate-buffered saline (PBS), 100 microl (1 x 10(7)) MB49 cells were infused and allowed to incubate in the bladder for 2 h followed intravesical mitomycin C (MMC) administration. The tumor formation rate, survival, gross hematuria, and bladder weight were determined as the outcome variables, and the pathology of the bladders was observed.</p><p><b>RESULTS</b>Instillation of MB49 tumor cells resulted in a tumor formation rates of 100% in all the pretreated groups while 0% in the control group without pretreatment. MMC significantly reduced the bladder weight as compared to PBS.</p><p><b>CONCLUSION</b>We have successfully established a stable, reproducible, and reliable orthotopic bladder cancer model in mice.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Tamaño de los Órganos
/
Patología
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Farmacología
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Vejiga Urinaria
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Neoplasias de la Vejiga Urinaria
/
Transformación Celular Neoplásica
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Mitomicina
/
Línea Celular Tumoral
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Modelos Animales de Enfermedad
/
Ratones Endogámicos C57BL
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2009
Tipo del documento:
Artículo
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