Preliminary identification and characterization of in vitro cultured bone marrow stromal cells for their bioactivity and osteogenic potential / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To induce the differentiation of bone marrow stromall cells (BMSCs) isolated from Beagles into osteoblasts in vitro and identify the osteogenic potential and bioactivity of the BMSCs.</p><p><b>METHODS</b>Primary cultured BMSCs isolated from Beagles were subcultured in mineralization medium to induce their differentiation into osteoblasts, whose morphological characteristics and proliferation status were observed by phase-contrast microscope. The osteogenic activity of the cells was evaluated with von Kossa staining of the mineralized nodules and determination of the alkaline phosphatase activity.</p><p><b>RESULT</b>BMSCs cultured in vitro showed obvious osteogenic capacity in DMEM. Von Kossa staining of the mineralized nodules and alkaline phosphatase detection of the passaged cells both yielded positive results.</p><p><b>CONCLUSION</b>BMSCs cultured in vitro contain osteogenic precursor cells, and the passaged cells possess osteogenic potential.</p>