Mesenchymal stem cells derived from human umbilical cord tissue modulate the secretion of antiplatelet antibody from splenocytes of ITP patients in vitro / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1372-1375, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-234231
ABSTRACT
The study was aimed to investigate the potential immunotherapeutical values of umbilical cord tissue-derived mesenchymal stem cells (UC-MSC) on patients with chronic idiopathic thrombocytopenic purpura (ITP). UC-MSC was cocultured in vitro with splenocytes isolated from ITP patients who experienced splenectomy. The level of IgG antiplatelet antibody (PAIgG) was determined by a competitive micro-enzyme-linked immunosorbent assay (ELISA) method. The proliferation of platelet-reactive CD4+ T lymphocytes was also measured in the presence of UC-MSCs. The results showed that UC-MSCs could stimulate the spontaneous secretion of PAIgG in supernatants; In the platelet-inducing condition, UC-MSC inhibited the production of PAIgG at a low ratio of 1 UC-MSC to 100 splenocytes, but promoted at a high proportion of 1 UC-MSC to 10 splenocytes. Moreover, UC-MSC exerted a suppressive effect on proliferation of platelet-reactive T helper cells in a dose-dependent manner. It is concluded that the UC-MSCs can regulate secretion of antiplatelet antibodies in vitro. Its concrete regulation mechanism and potential immunotherapeutical value are need to further study.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fisiología
/
Bazo
/
Cordón Umbilical
/
Plaquetas
/
Activación de Linfocitos
/
Linfocitos T CD4-Positivos
/
Púrpura Trombocitopénica Idiopática
/
Biología Celular
/
Proliferación Celular
/
Alergia e Inmunología
Límite:
Humanos
/
Recién Nacido
Idioma:
Chino
Revista:
Journal of Experimental Hematology
Año:
2008
Tipo del documento:
Artículo
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