Identification of the hemolytic transfusion reaction caused by lewis antibody using serological and molecular biological methods / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1192-1195, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-234269
ABSTRACT
To analyse the reason for one case of hemolytic transfusion reaction, antibodies in a patient's serum were identified using panel cells and Le (a-b-) phenotype cells, patient phenotype was identified by using anti-Le(a) and anti-Le(b) blood grouping reagents and the entire coding region of FUT3 gene was amplified by PCR and sequenced directly. The results showed that both IgM anti-Le(a) and anti-Le(b) antibodies were detected in patient's serum. Red cells was typed as Le (a-b-) phenotype and the FUT3 genotype was homozygote for non-functional le(59, 508) alleles. In conclusion, anti-Le(b) antibody can result in hemolytic transfusion reaction, FUT3 gene is homozygous for le(59, 508) allele resulting in Le (a-b-) phenotype.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Serología
/
Tipificación y Pruebas Cruzadas Sanguíneas
/
Alergia e Inmunología
/
Reacción a la Transfusión
/
Fucosiltransferasas
/
Genética
/
Genotipo
/
Enfermedades Hematológicas
/
Antígenos del Grupo Sanguíneo de Lewis
/
Anticuerpos
Tipo de estudio:
Estudio pronóstico
Límite:
Adulto
/
Femenino
/
Humanos
Idioma:
Chino
Revista:
Journal of Experimental Hematology
Año:
2008
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS