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Differentiation of bone marrow derived from mesenchymal stem cells into cardiomyocyte-like cells induced by co-culture with rat myocardial cells / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 1111-1115, 2008.
Artículo en Chino | WPRIM | ID: wpr-234288
ABSTRACT
The study was purposed to investigate the differentiation ability of mesenchymal stem cells (MSCs) into myocardial cells in vitro. Rat bone marrow-derived MSCs were labeled and co-cultured with neonatal rat cardiomyocytes (CM) for 5 - 7 days. The expression of cell surface antigens was detected by flow cytometry, and the expression of muscle-specific marker myosin and troponin T in labeled cells was detected by immunofluorescence. The results showed that in vitro cultured MSCs expressed CD90, CD44, CD105, CD54, not expressed CD34, CD45, CD31. After co-cultured with neonatal rat CM, labeled MSCs differentiated into cardiomyocyte-like cells expressing myosin and troponin T. It is concluded that MSCs can differentiate into cardiomyocyte-like cells when co-cultured with neonatal myocardial cells in vitro. In co-culture of two kind of cells in ratio of four to one showed obvious efficacy differentiating MSCs into CMs.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Células de la Médula Ósea / Diferenciación Celular / Células Cultivadas / Ratas Wistar / Técnicas de Cocultivo / Biología Celular / Miocitos Cardíacos / Células Madre Mesenquimatosas Límite: Animales Idioma: Chino Revista: Journal of Experimental Hematology Año: 2008 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Células de la Médula Ósea / Diferenciación Celular / Células Cultivadas / Ratas Wistar / Técnicas de Cocultivo / Biología Celular / Miocitos Cardíacos / Células Madre Mesenquimatosas Límite: Animales Idioma: Chino Revista: Journal of Experimental Hematology Año: 2008 Tipo del documento: Artículo