Identification of the new allele D18S1364-10 by sequence analysis / 中华医学遗传学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To identify a new allele by analyzing the polymorphism of D18S1364 in Power Plex(TM)16.</p><p><b>METHODS</b>An abnormal band was found in paternity test by short tandem repeat-PCR, and collected by gel extraction. Then the DNA was amplified, cloned and sequenced.</p><p><b>RESULTS</b>A fragment containing eight bases less than the minimal allele in the D18S1364 ladder, was found with the core sequence of (ATCT)₆(ATGT)₁(ATCT)₃. The allele was D18S1364-10.</p><p><b>CONCLUSION</b>The D18S1364-10 allele was found and reported for the first time in China.</p>