Establishment of allele specific primer polymerase chain reaction sequence-based typing and investigation of the polymorphism in exon 3 of HLA-DRB1 / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 639-643, 2010.
Article
en Zh
| WPRIM
| ID: wpr-234347
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish the allele specific primer polymerase chain reaction sequence-based typing (PCR-SBT) and investigate the polymorphism of exon 3 of human leukocyte antigen( HLA)-DRB1.</p><p><b>METHODS</b>The fragment containing exons 2 and 3 of HLA-DRB1 gene was amplified by group specific primers. The amplified products were digested by restriction enzymes and directly sequenced in both directions. The genotype was assigned by using Assign 3.5 SBT software.</p><p><b>RESULTS</b>The exon 3 sequences of HLA-DRB1*08:09 and HLA-DRB1*12:02:01 were identified for the first time. There were 27 polymorphic sites in exon 3 among the twenty-five HLA-DRB1 alleles, which was 9.56% of all nucleotides of exon 3. The method could discriminate the HLA-DRB1*14:01:01/14:54 ambiguous samples, and the HLA-DRB1*14:01:01 was identified in the Chinese population.</p><p><b>CONCLUSION</b>The PCR-SBT method for exon 3 of HLA-DRB1 from the present study was reliable and there were polymorphisms in exon 3 of HLA-DRB1.</p>
Texto completo:
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Índice:
WPRIM
Asunto principal:
Filogenia
/
Polimorfismo Genético
/
Datos de Secuencia Molecular
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Secuencia de Bases
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Antígenos HLA-DR
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Reacción en Cadena de la Polimerasa
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Exones
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Cartilla de ADN
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Evolución Molecular
/
Alelos
Límite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Medical Genetics
Año:
2010
Tipo del documento:
Article