Resveratrol activates AMPK and suppresses LPS-induced NF-kappaB-dependent COX-2 activation in RAW 264.7 macrophage cells / 대한해부학회지
Anatomy & Cell Biology
;
: 194-203, 2011.
Artículo
en Inglés
| WPRIM
| ID: wpr-23479
ABSTRACT
AMP-activated protein kinase (AMPK), an enzyme involved in energy homeostasis, regulates inflammatory responses, but its precise mechanisms are not fully understood. Recent evidence has shown that resveratrol (RES), an AMPK activator, reduces prostaglandin E2 production in lipopolysaccharide (LPS)-treated microglia. Here, we examined the effect of RES on nuclear factor kappa B (NF-kappaB) dependent cyclooxygenase (COX)-2 activation in LPS-treated RWA 264.7 macrophages. We found that treatment with RES increased AMPK activation. AMPK and acetyl CoA carboxylase phosphorylation were attenuated in cells treated with LPS+RES, compared to cells treated with LPS alone. RES inhibited tumor necrosis factor (TNF)-alpha and TNF receptor 1 in LPS-treated cells. Finally, RES inhibited LPS-induced NF-kappaB translocation into the nucleus and COX-2 expression. Moreover, the effects of 5-aminoimidazole-4-carboxamide ribose and compound C were consistent with the effects of RES in LPS-treated cells. Taken together, these results suggest that the anti-inflammatory action of RES in RAW 264.7 macrophages is dependent on AMPK activation and is associated with inhibition of the LPS-stimulated NF-kappaB-dependent COX-2 signaling pathway.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fosforilación
/
Acetil-CoA Carboxilasa
/
Ribosa
/
Estilbenos
/
Dinoprostona
/
FN-kappa B
/
Factor de Necrosis Tumoral alfa
/
Prostaglandina-Endoperóxido Sintasas
/
Receptores del Factor de Necrosis Tumoral
/
Microglía
Idioma:
Inglés
Revista:
Anatomy & Cell Biology
Año:
2011
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS