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Effect of folium panax quinquefolium saponins on apoptosis of cardiac muscle cells and apoptosis-related gene expression in rats with acute myocardial infarction / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine ; (12): 232-235, 2005.
Artículo en Chino | WPRIM | ID: wpr-234904
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of Folium Panax quinquefolium saponins (PQS) on apoptosis of cardiac muscle cells (CMCs) and apoptosis-related gene expression in rats with acute myocardial infarction (AMI).</p><p><b>METHODS</b>Fifty Wistar rats were randomly divided into 5 groups, 40 rats underwent coronary ligation of left anterior descending branch to establish AMI rats model, while to the other 10 rats in the sham-operation group, thoracotomy without coronary ligation was carried out. The AMI model rats in treated groups were treated with high-dosage PQS, low-dosage PQS, captopril respectively for 7 days, and those in the control group were treated with normal saline instead. Then, the apoptotic CMCs were labeled by TUNEL and the expression of Bcl-2 and Fas in cardiac muscle cells were determined by immunohistochemical methods.</p><p><b>RESULTS</b>The apoptotic index of CMCs in the model rats (46.48%) was significantly higher than that in the sham-operation group ( 1.03%, P<0.01 ). CMCs apoptosis rate in the low-dosage PQS group, the large-dosage group and the captopril group was 23.53 %, 17.58 % and 25.17 %, respectively, all were significantly lower than that in the sham-operation group (P<0.01). The expression of Bcl-2 gene was up-regulated and expression of Fas protein was down-regulated in the three treated groups.</p><p><b>CONCLUSION</b>PQS can inhibit CMCs apoptosis in AMI rats, downregulate Fas protein expression and up-regulate Bcl-2 protein expression, and has antagonistic effect in myocardial ischemic injury.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Patología / Farmacología / Saponinas / Sangre / Medicamentos Herbarios Chinos / Proteínas / Distribución Aleatoria / Química / Ratas Wistar / Apoptosis Límite: Animales Idioma: Chino Revista: Chinese Journal of Integrated Traditional and Western Medicine Año: 2005 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Patología / Farmacología / Saponinas / Sangre / Medicamentos Herbarios Chinos / Proteínas / Distribución Aleatoria / Química / Ratas Wistar / Apoptosis Límite: Animales Idioma: Chino Revista: Chinese Journal of Integrated Traditional and Western Medicine Año: 2005 Tipo del documento: Artículo