Establishment of a green fluorescent protein-labeled mouse model of adenoid cystic carcinoma / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 424-428, 2011.
Artículo
en Chino
| WPRIM
| ID: wpr-235028
ABSTRACT
<p><b>OBJECTIVE</b>To establish a novel nude mice model which can be visualized in real time and detected in a continuous and dynamic way for the development and metastasis of adenoid cystic carcinoma.</p><p><b>METHODS</b>Human adenoid cystic carcinoma cells, ACCM cell line, were infected with retroviral vector of pLEGFP-N1 and then screened for a single colony of ACCM-GFP cells. Cell proliferation and morphological analysis were conducted for ACCM and ACCM-GFP cells. Nude mice lingual carcinoma model was set up with ACCM-GFP cells injection and real time observation with fluorescence imaging on ACCM-GFP tumors was performed subsequently. Histological assay was analyzed for ACCM and ACCM-GFP tumors as well.</p><p><b>RESULTS</b>ACCM-GFP cells were able to express GFP stably in the long term. ACCM and ACCM-GFP cells showed no significant difference in cell proliferation and morphology, and no significant difference of histological characteristics in vivo could be found between ACCM and ACCM-GFP tumors. Tumor development could be monitored in real time with fluorescence imaging system in vivo.</p><p><b>CONCLUSION</b>GFP-expressing ACCM tumor model can be applied to detect and observe its development in the long term in a noninvasive, real time and dynamic way. It is also a kind of ideal in vivo mouse model for adenoid cystic carcinoma research.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Carcinoma Adenoide Quístico
/
Línea Celular Tumoral
/
Proteínas Fluorescentes Verdes
/
Proliferación Celular
/
Modelos Animales de Enfermedad
/
Ratones Endogámicos BALB C
/
Ratones Desnudos
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
/
Humanos
Idioma:
Chino
Revista:
West China Journal of Stomatology
Año:
2011
Tipo del documento:
Artículo
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