Establishment of palatal organ culture in vitro / 华西口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>The purpose of this study was to establish a palatal organ culture method and to investigate the palatogenesis in vitro.</p><p><b>METHODS</b>20 pregnant 14-day mice were killed, embryos were separated ascetically, and palatal shelves were dissected and placed on a modified Trowell's system. All explants were cultured 24 h and 48 h respectively. Finally, all explants were embedded and stained by Hematoxylin and Eosin.</p><p><b>RESULTS</b>All explants grew healthy. After incubation for 24 h, medial edge epithelium maintained, whereas after 48 h, medial edge epithelium disappeared, bilateral mesenchymal cells contacted, palates fused.</p><p><b>CONCLUSION</b>This method provides an effective way for investigating the etiology of cleft palate in vitro.</p>