Study on the in vitro effects of dexamethasone at different concentrations on the growth of murine embryonic palatal mesenchymal cell / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 206-224, 2011.
Artículo
en Chino
| WPRIM
| ID: wpr-235085
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of dexamethasone on the proliferation and apoptosis of embryonic palatal mesenchymal (EPM) cells, and chose a proper concentration of dexamethasone which can effect the ordinary growth of embryonic palatal mesenchymal cells.</p><p><b>METHODS</b>The primary EPM cells were isolated and cultured in vitro, then we did biological assay. EPM cells were treated with different concentration dexamethasone (1 x 10(-9), 1 x 10(-8), 1 x 10(-7) and 1 x 10(-6) mol x L(-1)) respectively. The proliferation of EPM cells was evaluated using MTT method. Apoptosis was examined quantitatively with fluorescein stain.</p><p><b>RESULTS</b>In the condition of blood serum's concentration at 10%, optical density step down following the raise of dexamethasone's concentration. The effect of dexamethasone got to a summit at 3 days. Inhibition rate of dexamethasone at 1 x 10(-6) mol x L(-1) was the highest.</p><p><b>CONCLUSION</b>Dexamethasone at 1 x 10(-6) mol x L(-1) can not only inhibit the growth of the EPM cells, but also will not lead to a large number of cells death. Therefore, this concentration can be used as a reference standard in future research. The most significant drug action time of dexamethason appears at the third day after administration, then the effect became weaken following the drug metabolism.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Técnicas In Vitro
/
Dexametasona
/
Células Cultivadas
/
Apoptosis
/
Proliferación Celular
/
Células Madre Mesenquimatosas
Límite:
Animales
/
Humanos
Idioma:
Chino
Revista:
West China Journal of Stomatology
Año:
2011
Tipo del documento:
Artículo
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