Application of quantum dots fluorescent probes in tissue of human tongue squamous cell carcinoma / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 175-178, 2011.
Artículo
en Chino
| WPRIM
| ID: wpr-235093
ABSTRACT
<p><b>OBJECTIVE</b>To study if quantum dots fluorescent probes can be applied to detect P53 protein and Bcl-2 protein in tissue of human tongue squamous cell carcinoma.</p><p><b>METHODS</b>By indirect immunofluorescence assay the same particle size quantum dots fluorescent probes were applied to detect P53 protein and Bcl-2 protein respectively. Different particle size quantum dots fluorescent probes were applied to detect P53 protein and Bcl-2 protein simultaneously in paraffin-embedded tissue section of human tongue squamous cell carcinoma under fluorescent microscope.</p><p><b>RESULTS</b>P53 protein and Bcl-2 protein can be combined with quantum dots fluorescent probes and specific fluorescene can be observed with ultraviolet light excited. P53 protein was mainly distributed in the nucleus, and Bcl-2 protein major in the cytoplasm. P53 protein and Bcl-2 protein can be combined with different particle size quantum dots fluorescent probes respectively in the same paraffin-embedded tissue section of human tongue squamous cell carcinoma and two kinds of fluorescene can be observed.</p><p><b>CONCLUSION</b>Quantum dots fluorescent probes can be applied to detect two kinds of specific protein in paraffin-embedded tissue section of human tongue squamous cell carcinoma.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Neoplasias de la Lengua
/
Carcinoma de Células Escamosas
/
Proteína p53 Supresora de Tumor
/
Puntos Cuánticos
/
Colorantes Fluorescentes
Límite:
Humanos
Idioma:
Chino
Revista:
West China Journal of Stomatology
Año:
2011
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS