Effect of Ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic-acid on human gastric cancer cells and its mechanism / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1345-1348, 2011.
Artículo
en Chino
| WPRIM
| ID: wpr-235127
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the apoptosis-inducing effect of Ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic-acid (5F), a compound isolated from Pteris semipinnata L (PsL), on human gastric cancer SGC7901 cells and explore its mechanism.</p><p><b>METHODS</b>The inhibitory effect of 5F on SGC7901 cells was observed by MTT assay and flow cytometry, and the changes of the expression of Bcl-2 and Bax in SGC7901 cells following 5F exposure were evaluated by Western blot analysis.</p><p><b>RESULTS</b>5F inhibited the proliferation of SGC7901 cells in a concentration- and time-dependent manner, and the cell apoptosis induced by 5F was confirmed by Annexin V-EGFP staining and caspase-3 activation assay. The cell apoptosis induced by 5F was associated with decreased Bcl-2 and increased Bax expressions.</p><p><b>CONCLUSION</b>5F exposure induces apoptosis in SGC7901 cells by activating mitochondrial apoptotic pathways.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Farmacología
/
Neoplasias Gástricas
/
Química
/
Apoptosis
/
Proteínas Proto-Oncogénicas c-bcl-2
/
Pteris
/
Línea Celular Tumoral
/
Proliferación Celular
/
Diterpenos
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2011
Tipo del documento:
Artículo
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