Identification of the IgE-binding epitopes in main dust mite allergen Der p 1 / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1183-1186, 2011.
Artículo
en Chino
| WPRIM
| ID: wpr-235168
ABSTRACT
<p><b>OBJECTIVE</b>To identify the IgE-binding epitopes in the allergen Der p 1 of main house dust mites, which can be recognized by the specific IgE in the sera from allergic individuals, and obtain a hypoallergen derived from the T-B epitope fused peptide for potential use in specific immunotherapy (SIT).</p><p><b>METHODS</b>Thirty-one peptides containing 15 amino acids each, which covered the full 222 amino acids of Der p 1 protein sequence, were synthesized on the cellulous membrane by solid-phase peptide (SPOTs) synthesis, with 8 overlapping amino acids between every two neighboring peptides. The membrane bearing the spots of the synthesized peptides were incubated with the allergic serum pools consisting of the sera from 5 allergic individuals. The membrane was then probed with HRP-conjugated anti-human IgE, followed by enhanced chemiluminescence (ECL) for visualization and gray scale analysis of the positive peptide spots.</p><p><b>RESULTS</b>Three strong IgE-binding epitopes were identified in the amino acid sequence of Der p 1 molecule, namely Ep1 (amino acids 85-99), Ep2 (amino acids 106-120) and Ep3 (amino acids 190-204).</p><p><b>CONCLUSION</b>The 3 IgE-binding epitopes (B cell epitopes) identified in Der p 1 confirm the presence of linear epitopes in Der p 1, suggesting the possibility of constructing T/B epitope-fused hypoallergens.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Sitios de Unión de Anticuerpos
/
Inmunoglobulina E
/
Cisteína Endopeptidasas
/
Datos de Secuencia Molecular
/
Secuencia de Aminoácidos
/
Linfocinas
/
Antígenos Dermatofagoides
/
Alergia e Inmunología
/
Proteínas de Artrópodos
/
Ácaros
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2011
Tipo del documento:
Artículo
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