Effect of different light intensity on Polygonum cuspidatum callus / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the effect of light intensity (LI) on the callus induction and resveratrol content of Polygonum cuspidatum.</p><p><b>METHOD</b>Taking leaves and stem segment of P. cuspidatum as explants, the callus was inducted and cultured under different LI. The growth status of callus was observed and analyzed. The content of resveratrol was determined by HPLC.</p><p><b>RESULT</b>The formation and growth of the stem segment callus were optimal under low light treatment (1340-1560 lx). The resveratrol content was 18.350 microg x g(-1) in fresh stem segment and 140.074 microg x g(-1) in dry stem segment, which was two times higher than that of wild P. cuspidatum.</p><p><b>CONCLUSION</b>Introduction and status of P. cuspidatum callus were obvious diverse under different LI, the resveratrol content of P. cuspidatum was enhanced under appropriate LI.</p>