Analysis on genetic stability in different development stages of Dendrobium huoshanense by RAPD / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To research the hereditary stability of Dendrobium huoshanense which were subcultured 7-8 times in the same tissue culture system.</p><p><b>METHOD</b>Using three primers of arbitrary decamer oligonucleotide sequences from 20 primers for DNA amplification, random amplified polymorphic DNA (RAPD) were performed.</p><p><b>RESULT</b>The genetic similarity coefficient was varied from 85.4% to 98.4%. The variation of protocorm, germination and monoleaf were rather more notable than that of bileaf and inflorescence.</p><p><b>CONCLUSION</b>The variation of the same pod is quite small. It is feasible to set up the rapid propagation system of D. huoshanense</p>