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Pathogenesis and immunogenicity of an avian H9N2 influenza virus isolated from human / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences ; (12): 530-536, 2011.
Artículo en Inglés | WPRIM | ID: wpr-235600
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the pathogenesis and immunogenicity of H9N2 influenza virus A/Guangzhou/333/99 (a reassortant of G1 and G9 viruses isolated from a female patient in 1999) in a mouse model of infection.</p><p><b>METHODS</b>Mice were infected with increasing virus titers. Viral load in the lungs and trachea was determined by EID50 assay. Pulmonary histopathology was assessed by hematoxylin-eosin staining. Anti-HI antibody titers and T-cell responses to viral HA were determined by ELISPOT and confirmed by flow cytometry.</p><p><b>RESULTS</b>Mice presented a mild syndrome after intranasal infection with A/Guangzhou/333/99 (H9N2) influenza virus. Virus was detected in the trachea and lungs of mice harvested on days 3, 6, and 9 post-infection. A T-cell response to viral HA was detected on day 6 and H9 HA-specific CD(4+) T-cells predominated. Seroconversion was detected after 14 days and antibody persisted for at least 28 weeks.</p><p><b>CONCLUSION</b>Our results suggest that H9N2 (A/Guangzhou/333/99) can replicate in the murine respiratory tract without prior adaptation, and both humoral and cell-mediated immunity play an important role in the immune response.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Bazo / Tráquea / Virología / Virulencia / Pruebas de Inhibición de Hemaglutinación / Linfocitos / Linfocitos T CD4-Positivos / Línea Celular / Interferón gamma / Infecciones por Orthomyxoviridae Tipo de estudio: Estudio de etiología Límite: Animales / Femenino / Humanos Idioma: Inglés Revista: Biomedical and Environmental Sciences Año: 2011 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Bazo / Tráquea / Virología / Virulencia / Pruebas de Inhibición de Hemaglutinación / Linfocitos / Linfocitos T CD4-Positivos / Línea Celular / Interferón gamma / Infecciones por Orthomyxoviridae Tipo de estudio: Estudio de etiología Límite: Animales / Femenino / Humanos Idioma: Inglés Revista: Biomedical and Environmental Sciences Año: 2011 Tipo del documento: Artículo