Cloning and expression analysis of a key device of HMGR gene involved in ginsenoside biosynthesis of Panax ginseng via synthetic biology approach / 药学学报
Acta Pharmaceutica Sinica
;
(12): 219-227, 2013.
Artículo
en Chino
| WPRIM
| ID: wpr-235680
ABSTRACT
3-Hydroxy-3-methylglutaryl coenzyme-A reductase (HMGR), the first enzyme of mavalonic acid pathway, is one of the key devices involved in ginsenoside biosynthesis based on synthetic biology approach. The open reading frame of a novel HMGR gene from Panax ginseng (PgHMGR2) was cloned and analyzed in this study. PgHMGR2-encoding protein showed 71.6% sequence similarity to a P. ginseng HMGR in GenBank. The full-length cDNA sequence of PgHMGR2 containing 1 770 bp, which encodes 589 amino acids, was cloned by RT-PCR strategy from P. ginseng. The bioinformatic analysis showed that PgHMGR2-encoding protein contained two transmembrane regions and the HMG_CoA_reductase domain, without signal peptide. The protein sequence of PgHMGR2 had the highest sequence similarity (99%) with Panax quinquefolius HMGR (GenBank accession No. ACV65036). The expression level of PgHMGR2 was the highest in flower based on a real-time PCR analysis, followed by leaf and root, and the lowest was in stem. The result will provide a foundation for exploring the molecular function of PgHMGR2 involved in ginsenoside biosynthesis based on synthetic biology approach in P. ginseng plants.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Filogenia
/
Plantas Medicinales
/
Regulación Enzimológica de la Expresión Génica
/
Sistemas de Lectura Abierta
/
Secuencia de Aminoácidos
/
Clonación Molecular
/
Homología de Secuencia de Aminoácido
/
Estructura Secundaria de Proteína
/
ADN Complementario
/
Tallos de la Planta
Idioma:
Chino
Revista:
Acta Pharmaceutica Sinica
Año:
2013
Tipo del documento:
Artículo
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