Evaluation and comparison of native and recombinant LipL21 protein-based ELISAs for diagnosis of bovine leptospirosis
Journal of Veterinary Science
;
: 99-101, 2012.
Artículo
en Inglés
| WPRIM
| ID: wpr-23570
ABSTRACT
A 21-kDa leptospiral lipoprotein (LipL21) was evaluated for its diagnostic potential to detect bovine leptospirosis by ELISA. Both native LipL21 (nLipL21) and recombinant LipL21 (rLipL21) proteins were tested and compared regarding diagnostic efficiency, and no statistically significant difference was observed. The sensitivity of rLipL21 ELISA for 62 microscopic agglutination test (MAT) positive sera was 100% and the specificity with 378 MAT negative sera was 97.09%. Thus, rLipL21 protein-based ELISA could be used as an alternative to MAT for the diagnosis of bovine leptospirosis.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Proteínas de la Membrana Bacteriana Externa
/
Proteínas Recombinantes
/
Ensayo de Inmunoadsorción Enzimática
/
Enfermedades de los Bovinos
/
Sensibilidad y Especificidad
/
Leptospira interrogans
/
Leptospirosis
/
Lipoproteínas
/
Anticuerpos Antibacterianos
/
Antígenos Bacterianos
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
Idioma:
Inglés
Revista:
Journal of Veterinary Science
Año:
2012
Tipo del documento:
Artículo
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