Adriamycin increases podocyte permeability: evidence and molecular mechanism / 中华医学杂志(英文版)

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the increased podocyte permeability by evidence of adriamycin (AD) and its molecular mechanism.</p><p><b>METHODS</b>In this study, we explored the direct effects of AD on cultured mouse podocytes and the potential protection effects of Dexamethasome (Dex).</p><p><b>RESULTS</b>After 24-hour AD (5 x 10(-7) mol/L) treatment, albumin passage through podocyte monolayers was increased by 2.27-fold (P < 0.01). AD caused a 62% decrease in Zonula Occluden-1 (ZO-1) protein (P < 0.05), suggesting that AD might increase podocyte permeability by disrupting tight junctions. Dex (1 x 10(-6) mol/L), co-administered with AD, protected podocytes from AD-induced increased albumin passage. This may be linked with an increased P-cadherin protein level to 1.93 fold of control (P < 0.01).</p><p><b>CONCLUSIONS</b>AD has a direct, detrimental effect on podocyte permeability, probably through disrupting tight junctions; Dex could protect against AD-induced high podocyte permeability by upregulating adherent protein P-cadherin.</p>