Experimental study of Porphyromonas gingivalis on the proliferation and apoptosis of vascular endothelial cells / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 343-346, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-235905
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of Porphyromonas gingivalis (Pg) on the proliferation and apoptosis of vascular endothelial cell and to identify the role of Pg in the pathogenesis of atherosclerosis.</p><p><b>METHODS</b>A cell culture model of vascular endothelial cell treated by Pg was used in vitro. The cells' ability of proliferation was measured by methyl thiazolyl tetrazolium assay, and cell cycle and the percentage of apoptosis with or without Pg invasion were examined by flow cytometry.</p><p><b>RESULTS</b>Invasion of Pg could inhibit the proliferation of vascular endothelial cells at 72 h (F = 786.68, P < 0.01), and induce apoptosis at 24 h (F = 1074.56, P < 0.01). In addition, the cell cycle was arrested at G1 phase by the invasion of Pg W83 at 24 h (F = 43.23, P < 0.01) and ATCC 33277 at 48 h (F = 66.72, P < 0.01).</p><p><b>CONCLUSIONS</b>Pg may aggregate the inflammation of the vascular endothelial cells through induction of apoptosis, which could be one of the pathologic mechanisms in atherosclerosis.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Células Cultivadas
/
Infecciones por Bacteroidaceae
/
Apoptosis
/
Porphyromonas gingivalis
/
Biología Celular
/
Células Endoteliales
/
Proliferación Celular
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Stomatology
Año:
2008
Tipo del documento:
Artículo
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