Atrial myocytes KChIP2 mRNA expression in rheumatic heart disease patients with atrial fibrillation / 中华心血管病杂志
Chinese Journal of Cardiology
; (12): 509-513, 2009.
Article
en Zh
| WPRIM
| ID: wpr-236465
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To detect the KChIP2 mRNA level in rheumatic heart disease patients with or without atrial fibrillation (AF) by real-time PCR.</p><p><b>METHODS</b>Right atrial appendage samples from rheumatic heart disease patients with (n = 17) or without AF (n = 13) were obtained during cardiac surgery. Total RNA was extracted from the atrial tissues, and the KChIP2 and Kv4.3 mRNA were detected by SYBR Green I real-time PCR with the GAPDH as the house keeping gene.</p><p><b>RESULT</b>The ratio of KChIP2/GAPDH (0.1468 +/- 0.0452 vs. 0.2200 +/- 0.0388, P<0.01) and the ratio of Kv4.3/GAPDH (0.3946 +/- 0.1826 vs. 0.5257 +/- 0.1427, P<0.05) were significantly lower in AF patients compared to non-AF patients.</p><p><b>CONCLUSION</b>Down-regulated atrial KChIP2 and Kv4.3 mRNA expressions in rheumatic heart disease patients with chronic AF might be one of the molecular bases responsible for the down-regulation of the I(to) current density of AF.</p>
Texto completo:
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Índice:
WPRIM
Asunto principal:
Cardiopatía Reumática
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Fibrilación Atrial
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ARN Mensajero
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Regulación hacia Abajo
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Miocitos Cardíacos
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Proteínas de Interacción con los Canales Kv
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Canales de Potasio Shal
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Genética
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Metabolismo
Límite:
Adult
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Female
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Humans
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Male
Idioma:
Zh
Revista:
Chinese Journal of Cardiology
Año:
2009
Tipo del documento:
Article