Comparative proteomic analysis of cerebral cortex between middle cerebral artery occlusion rats and normal controls / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 934-941, 2005.
Artículo
en Chino
| WPRIM
| ID: wpr-237047
ABSTRACT
In order to provide a complete picture of pathogenesis in cerebral ischemia, cerebral cortex in MCAO rats were analysed for alteration in their proteomes. Comparative proteome analysis was used to compare signal corresponding to individual cerebral cortex proteins on a two-dimensional gel between MCAO rats and the normal control (NC) group. After sample preparation, two-dimensional electroghoresis separated proteins were stained with Commassie Brilliant Blue. The image data were analyzed on a Dell computer using Image Master v 3.01 software. In cerebral cortex, 30 proteins were differentially expressed in MCAO rats compared with NC. There were 11 spots significantly increased, 15 spots significantly decreased and Adenylate kinase isoenzyme 1 was detected only in NC group, biliverdin reductase B, small inducible cytokine A4 [Precursor] only in MCAO group. Peroxiredoxin 2 divided into two points in MCAO6h group. In the end, this approach may lay a foundation for the further investigation of pathogenic mechanisms in cerebral ischmic injury.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Electroforesis en Gel Bidimensional
/
Distribución Aleatoria
/
Corteza Cerebral
/
Isquemia Encefálica
/
Química
/
Ratas Wistar
/
Infarto de la Arteria Cerebral Media
/
Proteoma
/
Perfilación de la Expresión Génica
/
Proteómica
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2005
Tipo del documento:
Artículo
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