The expression of recombinant protein of human interleukin-24 and its anti-tumor mechanism / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 719-724, 2005.
Artículo
en Chino
| WPRIM
| ID: wpr-237084
ABSTRACT
The hIL24 cDNA sequence was cloned into prokaryotic high expressive vector pET-21a(+) and recombinant hIL24 was expressed in E. coli with IPTG induction. The purified recombinant hIL24 exhibits following functions in HeLa cell inhibiting cell growth, inducing apoptosis, inducing PMBC to secrete IL-6, TNF-alpha, IFN-r and inhibiting blood vessel formation. Our preliminary results suggest that the apoptosis induced by rhIL24 is through down-regulating expression of anti-apoptosis factor Bcl-2 and activation of mitochondria apoptosis pathway.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Proteínas Recombinantes
/
Células HeLa
/
Interleucinas
/
Apoptosis
/
Proteínas Proto-Oncogénicas c-bcl-2
/
Alergia e Inmunología
/
Escherichia coli
/
Genética
/
Metabolismo
/
Mitocondrias
Límite:
Femenino
/
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2005
Tipo del documento:
Artículo
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