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Optimization of fermentation of recombinant human Endostatin (rh-Endostatin) expression in Escherichia coli / 生物工程学报
Chinese Journal of Biotechnology ; (12): 662-666, 2005.
Artículo en Chino | WPRIM | ID: wpr-237094
ABSTRACT
The fermentation process of recombinant human Endostatin expression in Escherichia coli BL21 (DE3) was studied. The effects of factors such as concentration of IPTG, induction time, cultivation temperature and feeding strategies were investigated. Beside that, by changing the temperature to 40 degrees C after induction, the high-density cultivation finished in a much shorter period. After 9 hours cultivation, the optical density (OD) at 600 nm reached 140 and the yield of inclusion body was 3 g/L. While E. coli system was used, protein with better activity and stability was obtained. The cost was much lower and the producing process was much steadier. It will meet the demands of the industrial production.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Tiogalactósidos / Proteínas Recombinantes / Ingeniería de Proteínas / Química / Endostatinas / Escherichia coli / Fermentación / Genética / Metabolismo Límite: Humanos Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2005 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Tiogalactósidos / Proteínas Recombinantes / Ingeniería de Proteínas / Química / Endostatinas / Escherichia coli / Fermentación / Genética / Metabolismo Límite: Humanos Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2005 Tipo del documento: Artículo