Celastrol down-regulates expression of P-Akt and cyclin D1 in HL-60 cells and induces apoptosis / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 898-902, 2010.
Artículo
en Chino
| WPRIM
| ID: wpr-237628
ABSTRACT
The aim of this study was to investigate the effect of Celastrol on induction of HL-60 cell apoptosis and its possible mechanism. The proliferative activity of HL-60 cells treated with 0.25 - 8.0 μmol/L of Celastrol for 24 - 72 hours was assayed by MTT method, the effects of Celastrol on apoptosis and cell cycle of HL-60 were detected by TUNEL staining and flow cytometry with Annexin V-FITC/PI double labeling, the expression of pAkt and cyclin D1 at protein and gene level in HL-60 cells treated with Celastrol were measured by Western blot and RT-PCR. The results showed that the Celastrol could obviously inhibit the proliferation of HL-60 cells in concentration-and time-dependent manners, the IC₅₀ value of Celastrol for 24 hours was 6.21 ± 0.242 μmol/L. The Celastrol concentration-dependently induced the apoptosis of HL-60 cells, accompanying with morphological changes of apoptotic cells, which may be related with arrest of cells in G₀/G₁ phase. The Celastrol suppressed the expression of pAkt and Cyclin D1 in HL-60 cells to a varying degree which showed obvious concentration-and time-dependent manners. It is concluded that the Celastrol inhibits the proliferation and induced the apoptosis of HL-60 cells. Its mechanism may be related with down-regulation of p-Act and cyclin D1 expressions.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Triterpenos
/
Regulación hacia Abajo
/
Regulación Leucémica de la Expresión Génica
/
Apoptosis
/
Células HL-60
/
Ciclina D1
/
Proteínas Proto-Oncogénicas c-akt
/
Metabolismo
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Experimental Hematology
Año:
2010
Tipo del documento:
Artículo
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