Study of reduction rat T-lymphocytes activation and proliferation by inhibition inducible co-stimulator gene expression / 中华外科杂志
Chinese Journal of Surgery
;
(12): 301-304, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-237797
ABSTRACT
<p><b>OBJECTIVE</b>To observe the change of activation and proliferation ability of rat T-lymphocytes after suppress ICOS gene expression by RNA interference.</p><p><b>METHODS</b>Four interference sites targeting at rat ICOS gene were designed and four pairs of oligonucleotide fragments were cloned into the pSilencer 4.1-CMV neo plasmid vectors then transfected into rat lymphocytes with cationic liposome. The expression of mRNA and protein of ICOS was detected by RT-PCR and flow cytometry. The alteration of lymphocyte proliferation ability was evaluated by mix lymphocyte reaction, and the secretion levels of IFN-gamma and IL-4 were measured by ELISA procedure.</p><p><b>RESULTS</b>After transfection, the expression of mRNA and protein of ICOS in test groups were lower than that in control groups (P < 0.05). The ability of T-lymphocytes in proliferation was poor and the levels of IFN-gamma and IL-4 were reduced with ICOS gene shut down.</p><p><b>CONCLUSIONS</b>RNA interference plasmid vector can suppress ICOS expression in rat T-lymphocytes significantly, and it may be useful for further study on transplantation immunity tolerance.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Activación de Linfocitos
/
Linfocitos T
/
Transfección
/
Antígenos de Diferenciación de Linfocitos T
/
Células Cultivadas
/
Interleucina-4
/
Interferón gamma
/
Ratas Sprague-Dawley
/
Interferencia de ARN
/
Proliferación Celular
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Surgery
Año:
2008
Tipo del documento:
Artículo
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