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Experimental research on human spermatozoa membrane proteins by two-dimensional gel electrophoresis / 中华男科学杂志
National Journal of Andrology ; (12): 504-506, 2003.
Artículo en Chino | WPRIM | ID: wpr-237988
ABSTRACT
<p><b>OBJECTIVES</b>To analyze human spermatozoa membrane proteins by two-dimensional gel electrophoresis and to provide a basis for drawing the protein map of normal human spermatozoa membrane proteins.</p><p><b>METHODS</b>Spermatozoa were purified by Percoll density centrifugation, and spermatozoa membrane proteins were analyzed by two-dimensional gel electrophoresis using isoelectric focusing and polyacrylamide gel electrophoresis.</p><p><b>RESULTS</b>About 800 protein spots could be identified by the imaging analysis system. The molecular weight and isoelectric point of most proteins were 20,000 to 100,000 and 3.0 to 7.0 respectively.</p><p><b>CONCLUSIONS</b>There are more than 800 types of proteins in the human spermatozoa membrane. The spermatozoa membrane protein can be identified with a certain precision by two-dimensional gel electrophoresis.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Espermatozoides / Procesamiento de Imagen Asistido por Computador / Electroforesis en Gel Bidimensional / Química / Focalización Isoeléctrica / Punto Isoeléctrico / Proteínas de la Membrana / Peso Molecular Límite: Humanos / Masculino Idioma: Chino Revista: National Journal of Andrology Año: 2003 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Espermatozoides / Procesamiento de Imagen Asistido por Computador / Electroforesis en Gel Bidimensional / Química / Focalización Isoeléctrica / Punto Isoeléctrico / Proteínas de la Membrana / Peso Molecular Límite: Humanos / Masculino Idioma: Chino Revista: National Journal of Andrology Año: 2003 Tipo del documento: Artículo