Experimental research on human spermatozoa membrane proteins by two-dimensional gel electrophoresis / 中华男科学杂志
National Journal of Andrology
;
(12): 504-506, 2003.
Artículo
en Chino
| WPRIM
| ID: wpr-237988
ABSTRACT
<p><b>OBJECTIVES</b>To analyze human spermatozoa membrane proteins by two-dimensional gel electrophoresis and to provide a basis for drawing the protein map of normal human spermatozoa membrane proteins.</p><p><b>METHODS</b>Spermatozoa were purified by Percoll density centrifugation, and spermatozoa membrane proteins were analyzed by two-dimensional gel electrophoresis using isoelectric focusing and polyacrylamide gel electrophoresis.</p><p><b>RESULTS</b>About 800 protein spots could be identified by the imaging analysis system. The molecular weight and isoelectric point of most proteins were 20,000 to 100,000 and 3.0 to 7.0 respectively.</p><p><b>CONCLUSIONS</b>There are more than 800 types of proteins in the human spermatozoa membrane. The spermatozoa membrane protein can be identified with a certain precision by two-dimensional gel electrophoresis.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Espermatozoides
/
Procesamiento de Imagen Asistido por Computador
/
Electroforesis en Gel Bidimensional
/
Química
/
Focalización Isoeléctrica
/
Punto Isoeléctrico
/
Proteínas de la Membrana
/
Peso Molecular
Límite:
Humanos
/
Masculino
Idioma:
Chino
Revista:
National Journal of Andrology
Año:
2003
Tipo del documento:
Artículo
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