Real-time fluorescent PCR for screening AZFc/DAZ microdeletions on the Y chromosome in male infertility patients / 中华男科学杂志
National Journal of Andrology
;
(12): 436-442, 2003.
Artículo
en Chino
| WPRIM
| ID: wpr-238003
ABSTRACT
<p><b>OBJECTIVE</b>To develop a real-time fluorescent PCR protocol suitable for the routine screening of AZFc/DAZ microdeletions on the Y chromosome in azoospermic and oligozoospermic male infertility patients.</p><p><b>METHODS</b>A set of real-time fluorescent PCR was established. Eighty-seven azoospermic and ligozoospermic patients undergoing ICSI in the IVF center and 30 azoospermic men undergoing testicular biopsy in the clinic of urology surgery were screened for AZFc/DAZ microdeletions of Y chromosome.</p><p><b>RESULTS</b>Eleven cases (9.4%) of AZFc/DAZ microdeletions were found in 117 cases of azoospermic and oligozoospermic patients by screening of realtime fluorescent PCR. Four cases (6.6%) were found in 61 oligozoospermic patients, and 7 cases (12.5%) were found in 56 azoospermic patients.</p><p><b>CONCLUSION</b>The real-time fluorescent PCR protocol presented in this study is an easy and reliable method for detection of AZFc/DAZ microdeletions on the Y chromosome, which yields identical results to those of the multiplex PCR.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Reacción en Cadena de la Polimerasa
/
Deleción Cromosómica
/
Proteínas de Unión al ARN
/
Cromosomas Humanos Y
/
Fluorescencia
/
Proteína 1 Delecionada en la Azoospermia
/
Genética
/
Infertilidad Masculina
/
Métodos
Tipo de estudio:
Estudio diagnóstico
/
Guía de Práctica Clínica
/
Estudio de tamizaje
Límite:
Humanos
/
Masculino
Idioma:
Chino
Revista:
National Journal of Andrology
Año:
2003
Tipo del documento:
Artículo
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