Molecular cloning and identification of calmegin gene in embryo and adult testes by cDNA microarray hybridization / 中华男科学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To identify gene differential expression in embryo and adult testes by cDNA microarray techniques.</p><p><b>METHODS</b>cDNA probes of embryo and adult testes were used to hybridize the cDNA microarray of human testis, and the clones of differential hybridization were sequenced and analyzed.</p><p><b>RESULTS</b>The calmegin (CLGN) gene which had been confirmed to be involved in spermatogenesis was found, and it exhibited more than 20-fold difference at expression level between adult and embryo human testes.</p><p><b>CONCLUSION</b>Genes differential expression in adult and embryo human testes can be identified by cDNA microarray hybridization.</p>