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IL-10 gene modification on immature dendritic cells induces antigen-specific tolerance in experimental autoimmune myocarditis / 中华心血管病杂志
Chinese Journal of Cardiology ; (12): 703-707, 2006.
Artículo en Chino | WPRIM | ID: wpr-238535
ABSTRACT
<p><b>OBJECTIVE</b>To investigate whether IL-10 gene modification on immature dendritic cells (iDC) could induce autoimmune tolerance in rat experimental autoimmune myocarditis (EAM).</p><p><b>METHODS</b>EAM was induced by cardiac myosin immunization on day 0 and day 7 in rats. A total of 2 x 10(6) mature DC (mDC), iDC, pcDNA3 transfected iDC, pcDNA3-IL-10 transfected iDC or PBS were injected intravenously at 5th immunization day. Three weeks later, echocardiography and HE staining were performed to observe the cardiac function and myocardial inflammation. Th1/Th2 cytokines were detected by ELISA and MHC-II molecules, costimulatory molecules were identified by flow cytometry. In vitro T lymphocyte proliferation assay and adoptive transfer of DCs were performed to determine the antigen specific tolerance induced by IL-10 gene modification on iDCs.</p><p><b>RESULTS</b>EAM rats treated with pcDNA3-IL-10 transfected iDC showed improved cardiac function and reduced inflammatory cells infiltration into myocardium. Moreover, lower Th1 and higher Th2-type response was induced, MHC-II and costimulatory molecules down-regulated and antigen specific immunological responses towards cardiac myosin inhibited in pcDNA3-IL-10-iDC treated EAM rats.</p><p><b>CONCLUSION</b>Treatment with IL-10 gene modified iDCs could ameliorates EAM by inducing Th2 polarization and down-regulation of MHC-II molecules and costimulatory molecule expressions.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Ratas Endogámicas Lew / Enfermedades Autoinmunes / Células Dendríticas / Células de la Médula Ósea / Animales Modificados Genéticamente / Terapia Genética / Línea Celular / Interleucina-10 / Alergia e Inmunología / Genética Tipo de estudio: Estudio pronóstico Límite: Animales Idioma: Chino Revista: Chinese Journal of Cardiology Año: 2006 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Ratas Endogámicas Lew / Enfermedades Autoinmunes / Células Dendríticas / Células de la Médula Ósea / Animales Modificados Genéticamente / Terapia Genética / Línea Celular / Interleucina-10 / Alergia e Inmunología / Genética Tipo de estudio: Estudio pronóstico Límite: Animales Idioma: Chino Revista: Chinese Journal of Cardiology Año: 2006 Tipo del documento: Artículo