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Intragastric administration of interferon-α-transformed Bifidobacterium promotes lymphocyte proliferation and maturation in mice / 南方医科大学学报
Journal of Southern Medical University ; (12): 326-332, 2015.
Artículo en Chino | WPRIM | ID: wpr-239183
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of intragastric administration of human interferon-α (hIFN-α)-transformed Bifidobacterium on immune functions of mice.</p><p><b>METHODS</b>The E.coli-Bifidobacterium shuttle expression vector containing hIFN-α gene was constructed and transformed into Bifidobacterium. The hIFN-α-transformed Bifidobacterium suspension (1010 /ml) was prepared after induction with 0.2% L-arabinose for hIFN-α expression and administered intragastrically in male Balb/C mice at the dose of 0.1 ml every other day for 2 weeks, with the mice receiving empty vector-transformed Bifidobacteria as the negative control and those having an equal volume of saline as the blank control. The percentages of mononuclear cell subsets in the thymus, spleen and blood were detected in the mice by flow cytometry, and the serum levels of IL-4, IL-12, IFN-γ and TNF-α were assayed using mouse cytokine FlowCytomix Kit.</p><p><b>RESULTS</b>The percentages of CD3⁺CD8⁺ and CD4⁺CD8⁺ cells in the thymus, CD3⁺CD4⁺, CD3⁺CD8⁺ and CD4⁺CD8⁺ cells in the spleen, and CD3⁺CD8⁺ cells in the blood all increased significantly in IFN group as compared with those in the negative and blank control groups (P<0.01 or 0.05). The serum level of IFN-γ also increased significantly (P<0.05) while IL-4 level remained unchanged in IFN group compared with those in the two groups.</p><p><b>CONCLUSION</b>Intragastric administration of hIFN-α-transformed Bifidobacterium promotes lymphocyte proliferation and maturation and increases the serum levels of Th1 cytokines in mice.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Bazo / Timo / Bifidobacterium / Sangre / Proteínas Recombinantes / Activación de Linfocitos / Interleucina-4 / Interferón gamma / Factor de Necrosis Tumoral alfa Límite: Animales / Humanos / Masculino Idioma: Chino Revista: Journal of Southern Medical University Año: 2015 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Bazo / Timo / Bifidobacterium / Sangre / Proteínas Recombinantes / Activación de Linfocitos / Interleucina-4 / Interferón gamma / Factor de Necrosis Tumoral alfa Límite: Animales / Humanos / Masculino Idioma: Chino Revista: Journal of Southern Medical University Año: 2015 Tipo del documento: Artículo