Studies on the changes of 5-hydroxymethyl furfuraldehyde content in Radix Rehmanniae steamed for different time / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To find the changing regularity of 5-Hydroxymethyl furfuraldehyde (5-HMF) content in Radix Rehmanniae steamed for different time.</p><p><b>METHOD</b>HPLC method was used, with C18 column (6 mm x 200 mm, 5 microm), a mixture of methanol-water (15 85) as the mobile phase. The flow rate was 1.0 mL x min(-1). The UV detection was setat 284 nm and the column temperature was set at 24 degrees C.</p><p><b>RESULT</b>The linear range was from 0.01 to 0.08 microg, r = 0.999 9 (n = 5). The average recovery was 100.2% and RSD was 2.7% (n = 5).</p><p><b>CONCLUSION</b>The 5-HMF content increases as the processing time prolongs in a certain time rang.</p>