Expression of JAK2V617F and MPLW515L/K mutation in 30 suspected cases of early myeloproliferative disorders / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 611-614, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-239973
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the prevalence of JAK2V617F and MPLW515L/K mutation in patients with slightly elevated platelets (BPC) or hemoglobin (Hb) not meeting the criteria of polycythemia vera (PV) or essential thrombocythemia (ET).</p><p><b>METHODS</b>Genomic DNA from bone marrow or blood mononuclear cells was screened with allele specific polymerase chain reaction (AS-PCR) for JAK2V617F and MPLW515L/K mutation. The history of thrombosis was assessed retrospectively by patients files.</p><p><b>RESULTS</b>Of 30 patients, 14 (46.7%) were positive for the JAK2V617F mutation, none of them had the MPLW515L/ K. Five of these 14 patients had a history of thrombosis. Follow-up results were available in 22 patients. Among them, 12 patients with JAK2V617F mutation turned out to be MPD in 6-24 months; only 2 out of 10 patients without this mutation evolved to MPD.</p><p><b>CONCLUSION</b>JAK2V617F mutation could be one of the diagnosis criteria of early MPD. No MPLW515L/K expression was found in early MPD.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Estudios de Seguimiento
/
Diagnóstico Precoz
/
Diagnóstico
/
Janus Quinasa 2
/
Receptores de Trombopoyetina
/
Genética
/
Metabolismo
/
Mutación
/
Trastornos Mieloproliferativos
Tipo de estudio:
Estudio diagnóstico
/
Estudio observacional
/
Estudio pronóstico
/
Factores de riesgo
/
Estudio de tamizaje
Límite:
Adolescente
/
Adulto
/
Anciano
/
Aged80
/
Femenino
/
Humanos
/
Masculino
Idioma:
Chino
Revista:
Chinese Journal of Hematology
Año:
2008
Tipo del documento:
Artículo
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