Influence of gene transduction mediated by lentivirus vector on gene expression of human CD34+ cord blood cells / 中华血液学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the influence of gene transduction mediated by lentivirus vector on human CD34+ cord blood cell (CBCs) gene expression.</p><p><b>METHODS</b>CD34+ cells were isolated and transduced with the third-generation self-inactivating ( SIN) lentiviral vector carrying green fluorescent protein (GFP). The total RNA from transduced cells was extracted and the differences of genotypes between the transduced and non-transduced CD34+ cells were determined with cDNA microarray analysis.</p><p><b>RESULTS</b>In 23000 genes two were upregulated and six downregulated. These changes were not confirmed by semi-quantitative RT-PCR method.</p><p><b>CONCLUSIONS</b>Lentiviral vector used in this study do not influence significantly on the gene expression of CD34+ CBCs, and the vector system may be a useful and safe one in clinical gene therapy.</p>