Interaction of Flightless I with Nup88 and Importin β / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1247-1254, 2015.
Artículo
en Chino
| WPRIM
| ID: wpr-240559
ABSTRACT
High expression of Fightless I (FLII) is associated to multiple tumors. Based on our previous study that FLII might be involved in the nuclear export, we assessed the possible interaction of FLII with the nuclear envelop associating proteins Importin β and Nup88. We first constructed GST-FLII, GST-LRR recombinant plasmids and transformed them into the Rosetta strain to produce GST-FLII, GST-LRR fusion protein. After purification of these proteins, GST-pull down, as well as co-immunoprecipitation, were used to test the interaction of FLII with Importin β and Nup88. FLII interacted with Importin β and Nup88, and FLII LRR domain is responsible for these interactions. Thus, FLII may play a role in nuclear export through interaction with Importin β and Nup88.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Proteínas Recombinantes de Fusión
/
Receptores Citoplasmáticos y Nucleares
/
Beta Carioferinas
/
Proteínas de Complejo Poro Nuclear
/
Metabolismo
/
Proteínas de Microfilamentos
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2015
Tipo del documento:
Artículo
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