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Differential expression of ODF1 in human ejaculated spermatozoa and its clinical significance / 中华男科学杂志
National Journal of Andrology ; (12): 891-894, 2009.
Artículo en Chino | WPRIM | ID: wpr-241236
ABSTRACT
<p><b>OBJECTIVE</b>To compare the expressions of ODF1 (outer dense fiber of the sperm tail 1) in ejaculated spermatozoa from normozoospermic and asthenozoospermic men with low sperm motility.</p><p><b>METHODS</b>Semen analyses were performed on the semen samples obtained from normozoospermic (n=20) and asthenozoospermic (n=20) volunteers according to the WHO criteria. To rule out the contamination of germ cells and leucocytes, the human ejaculated spermatozoa were purified by a discontinuous Percoll density gradient centrifugation. RT-PCR and Western blot were used to detect the expressions of ODF1 in the spermatozoa from the two groups.</p><p><b>RESULTS</b>RT-PCR showed that the expression of ODF1 mRNA was significantly lower in the spermatozoa from the asthenozoospermic patients than in those from the normozoospermic men (1.35 +/- 0.25 vs. 2.79 +/- 0.28, P < 0.05). Western blot confirmed the results from RT-PCR and revealed an obviously decreased expression of ODF1 in the spermatozoa of the asthenozoospermic patients, with statistically significant difference from the normozoospermic group (1.44 +/- 0.26 vs. 3.64 +/- 0.34, P < 0.05).</p><p><b>CONCLUSION</b>The expression of ODF1 was significantly decreased in the ejaculated spermatozoa of asthenozoospermic men, which might be responsible for low sperm motility.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Motilidad Espermática / Espermatozoides / Astenozoospermia / Proteínas de Choque Térmico / Metabolismo Límite: Humanos / Masculino Idioma: Chino Revista: National Journal of Andrology Año: 2009 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Motilidad Espermática / Espermatozoides / Astenozoospermia / Proteínas de Choque Térmico / Metabolismo Límite: Humanos / Masculino Idioma: Chino Revista: National Journal of Andrology Año: 2009 Tipo del documento: Artículo