Authentication of Zanthexylum bungeanum Maxin population and adulterants by analysis of rDNA ITS sequences / 药学学报
Acta Pharmaceutica Sinica
;
(12): 80-86, 2005.
Artículo
en Chino
| WPRIM
| ID: wpr-241333
ABSTRACT
<p><b>AIM</b>To study the difference of rDNA ITS sequences between Zanthexylum bungeanum populations and their adulterants in main habitants of China so as to provide molecular markers for identifying Zanthexylum bungeanum populations against adulterants.</p><p><b>METHODS</b>rDNA ITS regions (including ITS-1, 5.8S and ITS-2) of 7 populations of Zanthexylum bungeanum which are separate located in Gansu, Shanxi, Sichuan, Hebei provinces, and 3 adulterants were sequenced by PCR products sequencing method or clone sequencing method.</p><p><b>RESULTS</b>The sequences of rDNA ITS region of Zanthexylum bungeanum were reported for the first time, and the sequences of ITS region ranged from 619 to 620 bp, and the length difference amoung Zanthexylum bungeanum and their adulterants is 4 bp. There are 15 variable sites, 12 informative sites and 3 authenticable sites among Zanthexylum bungeanum populations. The difference of rDNA ITS regions amoung Zanthexylum bungeanum and their adulterants is obvious, the number of variable sites is 71.</p><p><b>CONCLUSION</b>The difference of rDNA ITS sequences can be used to authenticate accurately the populations of Zanthexylum bungeanum and their adulterants. These populations of Z. bungeanum which have close relationship always distribute in near geographic areas. The characteristics of rDNA ITS sequence can be used as good markers for authenticating Zanthexylum bungeanum populations form their adulterants.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Filogenia
/
Plantas Medicinales
/
Especificidad de la Especie
/
Datos de Secuencia Molecular
/
Secuencia de Bases
/
China
/
Contaminación de Medicamentos
/
Clasificación
/
Análisis de Secuencia de ADN
/
Ecosistema
Tipo de estudio:
Estudio pronóstico
País/Región como asunto:
Asia
Idioma:
Chino
Revista:
Acta Pharmaceutica Sinica
Año:
2005
Tipo del documento:
Artículo
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