Liquid chromatography frontal analysis of the protein binding of glimepiride / 药学学报

ABSTRACT

<p><b>AIM</b>To study the protein binding of glimepiride.</p><p><b>METHODS</b>An HPLC-FA method is performed by using Pinkerton GFF II-S5-80 internal-surface reversed-phase silica support (150 mm x 4.6 mm ID, 5 microm) at pH 7.4 in a 67 mmol x L(-1) isotonic sodium phosphate buffer at 37 degree C. Other conditions included flow rate of 0.2 mL x min(-1), UV detection at wavelength 230 nm and injection volume 900 microL.</p><p><b>RESULTS</b>Nonlinear regression parameter estimation was used for the association constant measurement of glimepiride to both primary and secondary sites, which were 5.1 (micromol x L(-1)-1 and 1 for K1 and n1, and 0.017 (micromol x L(-1))-1 and 7 for K2 and n2, respectively.</p><p><b>CONCLUSION</b>The method is shown to be suitable for investigation of protein binding of glimepiride.</p>